In a groundbreaking study published in Nature Plants, researchers have unveiled a novel biochemical link connecting photorespiration, a fundamental metabolic pathway in plants, to DNA methylation via formate as a critical one-carbon donor. This discovery not only deepens our understanding of plant physiology but also highlights a previously unrecognized integration between central carbon metabolism and epigenetic regulation, which could revolutionize agricultural biotechnology and plant breeding strategies.
Photorespiration, long considered a somewhat wasteful process overshadowing photosynthesis, is now taking center stage as scientists decode its broader cellular significance. During photorespiration, plants recycle a toxic byproduct of oxygenation by the enzyme Rubisco, ultimately producing serine and glycine among other metabolites. Yet, until now, the connection between photorespiratory intermediates and epigenetic modifications remained elusive. The new research fills this gap by demonstrating that formate generated through photorespiratory metabolism acts as a pivotal one-carbon unit supplier for DNA methylation.
DNA methylation, an epigenetic modification involving the addition of methyl groups predominantly to cytosine bases, plays crucial roles in gene expression regulation, development, and stress responses in plants. It requires one-carbon units provided by folate-dependent pathways, which had been primarily linked to folate cycles and amino acid metabolism. The current study unravels an alternative and complementary one-carbon source derived directly from photorespiration through formate, bridging metabolic processes with gene regulatory mechanisms.
To uncover this connection, the researchers employed a combination of isotope tracing, metabolomics, and epigenomic profiling in Arabidopsis thaliana, revealing that photorespiratory flux impacts nuclear DNA methylation patterns via a formate-dependent mechanism. Under normal and photorespiratory-enhanced conditions, elevated levels of formate were observed, coinciding with increased DNA methylation at specific genomic loci related to photosynthetic and stress-responsive genes. This inverse relationship with gene expression suggests an adaptive epigenetic response modulated through metabolic cues.
Further mechanistic analysis indicated that mitochondrial and peroxisomal activities of photorespiration converge to generate formate pools, which subsequently fuel cytosolic one-carbon metabolism intersecting with folate and methyl donor pathways. This metabolic interplay enables the incorporation of formate-derived methyl units into S-adenosylmethionine (SAM), the universal methyl group donor essential for DNA methyltransferases. Thus, photorespiration indirectly contributes to the epigenetic landscape by modulating SAM availability.
The implications of this discovery extend beyond basic science. It reshapes our conceptual framework of how environmental factors influencing photorespiration—for instance, high light intensity, temperature variations, or drought stress—can fine-tune epigenetic modifications through metabolic intermediates. This metabolite-epigenome crosstalk equips plants with a dynamic regulatory toolkit to adjust gene expression programs rapidly in response to fluctuating environments, potentially enhancing resilience.
Moreover, manipulating photorespiratory pathways could become a strategic approach to epigenetic engineering. By controlling formate flux, plant breeders or synthetic biologists could target desirable DNA methylation patterns, improving traits such as yield, stress tolerance, or developmental timing without direct genome editing. This metabolic lever provides a non-transgenic route to customize epigenomes with potential applications in sustainable agriculture.
The study also challenges the traditional view of photorespiration as merely a mitigating or compensatory mechanism. Instead, it portrays photorespiration as an active metabolic hub integrating carbon and one-carbon metabolism with gene regulatory systems, positioning it as a key determinant of cellular homeostasis. This paradigm shift invites new research into other potential metabolite-signaling roles emerging from core pathways previously thought to have limited regulatory impact.
This integrative perspective fosters interdisciplinary efforts combining plant physiology, systems biology, and epigenetics to uncover more layers of metabolic-epigenomic interplay. The technologies leveraged—such as advanced mass spectrometry-based metabolomics and bisulfite sequencing—exemplify the power of combining high-resolution molecular tools to interrogate complex biological phenomena.
Future research directions prompted by these findings include exploring whether similar formate-dependent epigenetic mechanisms exist in crop species and how they influence phenotypic plasticity under various abiotic stresses. Additionally, dissecting the transport and compartmentalization dynamics of formate within subcellular organelles might reveal regulatory nodes for metabolic channeling toward epigenetic modifications.
On a broader scale, the metabolic link to epigenetics might extend to other one-carbon metabolites and their sources, implying a more widespread metabolic network influencing epigenomic states across plant lineages. Understanding these connections will be instrumental in developing next-generation strategies for crop improvement aimed at stability and productivity amidst climate change challenges.
In summary, the research by Hankofer, Ghirardo, Obermaier, and colleagues represents a landmark advance in plant biology, illuminating a new dimension of metabolic control over the epigenome via photorespiratory formate. This multifaceted relationship opens exciting avenues for botanical science, agriculture, and synthetic biology, with the promise of novel solutions to global food security and environmental adaptation through metabolic-epigenetic engineering.
Subject of Research: The research focuses on elucidating the biochemical and molecular link between photorespiration and DNA methylation in plants, emphasizing formate as a key one-carbon metabolic intermediate.
Article Title: Photorespiration is linked to DNA methylation by formate as a one-carbon source
Article References:
Hankofer, V., Ghirardo, A., Obermaier, L. et al. Photorespiration is linked to DNA methylation by formate as a one-carbon source. Nat. Plants (2026). https://doi.org/10.1038/s41477-026-02222-x
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