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Home Science News Cancer

Johns Hopkins researchers identify key markers in pancreatic cancer progression using a new analysis pipeline

August 29, 2024
in Cancer
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FOR IMMEDIATE RELEASE

Using a new workflow that integrates spatial transcriptomics and machine learning for imaging analysis and integration with single-cell datasets, researchers at the Johns Hopkins Kimmel Cancer Center have identified novel molecular and cellular markers in the development of one of the most aggressive, deadly pancreatic cancers: pancreatic ductal adenocarcinoma (PDAC). 

A novel computational pipeline

Credit: Johns Hopkins Kimmel Cancer Center

FOR IMMEDIATE RELEASE

Using a new workflow that integrates spatial transcriptomics and machine learning for imaging analysis and integration with single-cell datasets, researchers at the Johns Hopkins Kimmel Cancer Center have identified novel molecular and cellular markers in the development of one of the most aggressive, deadly pancreatic cancers: pancreatic ductal adenocarcinoma (PDAC). 

PDAC arises from precancerous lesions in the pancreas. One of these lesion types, pancreatic intraepithelial neoplasias (PanINs), can appear in the pancreas years before they progress to invasive cancer. Because PanINs are so small, they cannot be detected by conventional clinical imaging tests. 

Previous analysis methods, such as bulk sequencing and single-cell sequencing, can capture gene expression of cancer cells and other cell types in the tumor microenvironment. However, what is missing is the spatial relationships among these types of cells within and around tumors, says senior study co-author Luciane Kagohara, Ph.D., an assistant professor of oncology at the Johns Hopkins University School of Medicine. 

Elana Fertig, Ph.D., professor of oncology, director of the oncology quantitative sciences division, and co-director of the Convergence Institute and the Single-Cell Training and Analysis Center at the Johns Hopkins University School of Medicine, was also a senior study co-author. 

Using spatial transcriptomics — a technique used to measure and map gene expression in a tissue section — the researchers developed a three-way analysis pipeline to map changes in gene expression from nine patients in 14 PanINs, including five rare, high grade PanINs. The machine learning tools used for imaging analyses (CODA) and for the integration with PDAC single-cell datasets, through the innovative multiomics integration methods CoGAPS (coordinated gene activity in pattern sets) and projectR, were developed in previous studies at Johns Hopkins. The investigators have made their data and code available to other researchers to uncover further insights into pancreatic cancer and, through open-source tools, to adapt the analysis methods for spatial transcriptomics for biomedical research at large. 

Results of their work, published Aug. 7 in Cell Systems, provide new insights into the gene expression and spatial distribution of different types of cells in the precancerous environment around PanINs. The findings are key to understanding how PanINs progress to PDAC, laying a foundation for future early detection of this and other types of pancreatic cancer. 

The three-way analysis revealed that some key features of pancreatic cancer were present in PanINs. 

“When we looked at the progression of normal to high grade PanIN lesions, we found that cell proliferation gradually increases while inflammatory signaling decreases, which can be important to understand the intrinsic low immunogenicity of these tumors,” says Kagohara. This suggests that the cells in and around PanINs are already creating a more immunosuppressive environment before the invasive PDAC is fully developed, she says. 

The findings also revealed spatial differences in the cells. “We found that cancer-associated fibroblasts, which play a major role in the biology of PDAC and their response to treatments, were already present at the premalignant stage, consistent with previous research in animal models of pancreatic cancer but not previously observed in humans,” says Kagohara. 

“PanINs are pretty small — less than 1 millimeter in size — so we were very surprised that even using very few cells, we were able to detect strong signatures from those lesions,” she adds. “That’s very important in cancer because, for example, if we want to understand immune responses to treatments, we need to know which immune cells are closer to or further from the tumor. But we also need to understand if there are other cell types that are blocking these communications by forming a physical barrier or interacting with the neoplastic cells.” 

Beyond the main findings, the study highlights the power of computational tools to further understanding of human disease.

Spatial transcriptomics is a very powerful technology for generating data, says Kagohara, but the right computational tools are needed to analyze it. “With the right tools, you can enhance the results that you can extract from these novel technologies,” she says. 

To encourage more collaboration and analyses, the study’s data is available on GEO (GSE254829), and the code is available on GitHub.

“As we generate more and more data on PanINs, the idea is to identify molecular signatures for the development of early detection tests,” says Kagohara. “This is one of the first studies to generate a resource open to other researchers in the search for these early markers in PanINs so we can learn more about how the spatial distribution of molecular and cellular signatures affect the progression of pancreatic cancer and the response to treatments.”

Other study co-authors include Alexander Bell, Jacob Mitchell, Ashley Kiemen, Melissa Lyman, Kohei Fujikura, Jae Lee, Eron Coyne, Sarah Shin, Sushma Nagaraj, Atul Deshpande, Pei-Hsun Wu, Dimitrios Sidiropoulos, Rossin Erbe, James Chell, Lauren Ciotti, Jacquelyn Zimmerman, Denis Wirtz, Won Jin Ho, Neeha Zaidi, Elizabeth Thompson, Elizabeth Jaffee and Laura Wood of Johns Hopkins. Researchers from 10x Genomics of Pleasanton, California, contributed to the work. 

The work was supported by a Johns Hopkins Discovery Award and multiple grants from the National Institutes of Health, the Sol Goldman Pancreatic Cancer Research Center, Stand Up to Cancer, Lustgarten Foundation, the Emerson Collective Cancer Research Fund, the Allegheny Health Network, Susan Wojcicki and Dennis Troper and the Rolfe Pancreatic Cancer Foundation. 

Jaffee reports other support from Abmeta Therapeutics, personal fees from Genocea, personal fees from Achilles Therapeutics, personal fees from Dragonfly Therapeutics, personal fees from Candel Therapeutics, other support from the Parker Institute for Cancer Immunotherapy, grants and other support from the Lustgarten Foundation, personal fees from Carta, grants and other support from Genentech, grants and other support from AstraZeneca, personal fees from NextCure, and grants and other support from Break Through Cancer outside of the submitted work. Fertig is a consultant to Mestag Therapeutics and is on the scientific advisory board of Viosera Therapeutics/Resistance Bio. Ho reports patent royalties from Rodeo/Amgen and speaking/travel honoraria from Exelixis and Standard BioTools.



Journal

Cell Systems

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