Saturday, September 6, 2025
Science
No Result
View All Result
  • Login
  • HOME
  • SCIENCE NEWS
  • CONTACT US
  • HOME
  • SCIENCE NEWS
  • CONTACT US
No Result
View All Result
Scienmag
No Result
View All Result
Home Science News Science Education

Epitope binning-seq: A game-changer in antibody drug discovery

June 10, 2024
in Science Education
Reading Time: 4 mins read
0
Enhancing Antibody Drug Discovery with Epitope Binning-seq Technology
65
SHARES
591
VIEWS
Share on FacebookShare on Twitter
ADVERTISEMENT

Monoclonal antibodies are crucial therapeutics due to their high specificity and binding affinity for epitopes (the precise sites where an antibody attaches itself to an antigen, triggering an immune response). Since the therapeutic efficacy of antibodies is closely related to their target epitopes, epitope characterization is essential for understanding antibody functionality. However, the traditional methods used for studying epitopes are slow and labor-intensive. Therefore, there is a pressing need for more efficient approaches that can help map epitopes with precision and speed, ultimately accelerating the development of next-generation therapies.

Enhancing Antibody Drug Discovery with Epitope Binning-seq Technology

Credit: Tokyo Tech

Monoclonal antibodies are crucial therapeutics due to their high specificity and binding affinity for epitopes (the precise sites where an antibody attaches itself to an antigen, triggering an immune response). Since the therapeutic efficacy of antibodies is closely related to their target epitopes, epitope characterization is essential for understanding antibody functionality. However, the traditional methods used for studying epitopes are slow and labor-intensive. Therefore, there is a pressing need for more efficient approaches that can help map epitopes with precision and speed, ultimately accelerating the development of next-generation therapies.

To bridge this gap, researchers from Tokyo Institute of Technology embarked on groundbreaking research, resulting in the development of a novel platform called Epitope Binning-seq. Their findings were published on 28 May, 2024 in Volume 7 of Communications Biology.  Epitope Binning-seq, evaluates epitope similarity using genetically encoded query antibodies (qAbs) on antigen-expressing cells and next-generation sequencing (NGS), allowing simultaneous evaluation of multiple qAbs without individual purification.

In this study, query antibodies (qAbs) displayed on the surface of cells expressing HER2 were employed. By introducing fluorescently labeled reference antibodies (rAbs) and using flow cytometry analysis, researchers could distinguish between cells where the qAbs masked the epitopes and cells where they did not. Some qAbs effectively blocked the binding of rAb to the antigen, resulting in a population of cells with no rAb binding, termed the rAb-negative population. Conversely, other qAbs allowed the rAb to bind to the antigen, creating a population of cells with rAb binding, known as the rAb-positive population.

Giving further insights into their study, Associate Professor Tetsuya Kadonosono, the corresponding author of the study, says, “The differential binding behavior served as a basis for evaluating epitope similarity between different antibodies. We performed NGS analysis on the sorted rAb-negative populations to identify and group similar qAbs into epitope bins. This comprehensive approach enabled efficient analysis of a large number of antibodies and classification based on their epitope specificity.”

The results were quite promising. Epitope Binning-seq accurately classified antibodies into distinct epitope bins, providing valuable insights into their binding patterns. In experiments using model antibodies pertuzumab and trastuzumab, the method accurately identified and enriched specific qAbs, even detecting clones at very low initial abundances. The platform classified 14 qAbs into correct epitope bins, with high precision. These findings showcase Epitope Binning-seq’s potential to streamline early antibody drug development by evaluating millions of qAbs simultaneously, accelerating the identification of promising therapeutic candidates.

The benefits of this platform are significant. Epitope Binning-seq streamlines epitope comparison, enabling rapid identification of antibodies with similar binding patterns, potentially advancing targeted and effective antibody-based therapies. The method’s large-scale evaluation can transform antibody characterization, making it possible to assess millions of qAbs simultaneously.

“The development of Epitope Binning-seq represents a major advancement in antibody drug discovery. By providing a rapid, comprehensive, and cost-effective method for evaluating antibody epitope similarity, this novel platform overcomes the limitations of traditional epitope analysis techniques,” concludes Kadonosono.

The promising results of this study highlight the potential of this method to transform early antibody drug development and antibody affinity maturation, paving the way for more effective therapeutic strategies in the future.

 

 

###

About Tokyo Institute of Technology

Tokyo Tech stands at the forefront of research and higher education as the leading university for science and technology in Japan. Tokyo Tech researchers excel in fields ranging from materials science to biology, computer science, and physics. Founded in 1881, Tokyo Tech hosts over 10,000 undergraduate and graduate students per year, who develop into scientific leaders and some of the most sought-after engineers in industry. Embodying the Japanese philosophy of “monotsukuri,” meaning “technical ingenuity and innovation,” the Tokyo Tech community strives to contribute to society through high-impact research.

 

 

 

 



Journal

Communications Biology

DOI

10.1038/s42003-024-06363-7

Method of Research

Experimental study

Subject of Research

Not applicable

Article Title

Epitope binning for multiple antibodies simultaneously using mammalian cell display and DNA sequencing

Article Publication Date

28-May-2024

Share26Tweet16
Previous Post

Association found between media diet and science-consistent beliefs about climate change

Next Post

Super-chilled brain cell molecules reveal how epilepsy drug works

Related Posts

blank
Science Education

Thai Medical Students: Research Skills and Motivations

September 5, 2025
blank
Science Education

Impact of Education on Pharmaceutical Promotion in Pakistan

September 5, 2025
blank
Science Education

Early Clinical Experience Sparks Medical Student Growth

September 5, 2025
blank
Science Education

Revolutionizing Blood Cell Education with AI Technology

September 4, 2025
blank
Science Education

Extensive Reading Boosts EFL Undergraduates’ Writing Skills

September 4, 2025
blank
Science Education

Authors and Reviewers Support Transparent Peer Review Pilot

September 4, 2025
Next Post
AMPA receptor

Super-chilled brain cell molecules reveal how epilepsy drug works

  • Mothers who receive childcare support from maternal grandparents show more parental warmth, finds NTU Singapore study

    Mothers who receive childcare support from maternal grandparents show more parental warmth, finds NTU Singapore study

    27544 shares
    Share 11014 Tweet 6884
  • University of Seville Breaks 120-Year-Old Mystery, Revises a Key Einstein Concept

    960 shares
    Share 384 Tweet 240
  • Bee body mass, pathogens and local climate influence heat tolerance

    643 shares
    Share 257 Tweet 161
  • Researchers record first-ever images and data of a shark experiencing a boat strike

    510 shares
    Share 204 Tweet 128
  • Warm seawater speeding up melting of ‘Doomsday Glacier,’ scientists warn

    313 shares
    Share 125 Tweet 78
Science

Embark on a thrilling journey of discovery with Scienmag.com—your ultimate source for cutting-edge breakthroughs. Immerse yourself in a world where curiosity knows no limits and tomorrow’s possibilities become today’s reality!

RECENT NEWS

  • Novel Antibody-Drug Conjugate Demonstrates Promising Efficacy in EGFR-Mutated NSCLC Patients
  • Phase II Study Finds Iza-Bren Plus Osimertinib Achieves 100% Response Rate in EGFR-Mutated NSCLC
  • COMPEL Study Finds Adding Chemotherapy to Osimertinib After Progression Enhances Progression-Free Survival in EGFR-Mutated NSCLC
  • Study Confirms Accuracy of AI Lung Cancer Risk Model Sybil in Predominantly Black Patients at Urban Safety-Net Hospital

Categories

  • Agriculture
  • Anthropology
  • Archaeology
  • Athmospheric
  • Biology
  • Blog
  • Bussines
  • Cancer
  • Chemistry
  • Climate
  • Earth Science
  • Marine
  • Mathematics
  • Medicine
  • Pediatry
  • Policy
  • Psychology & Psychiatry
  • Science Education
  • Social Science
  • Space
  • Technology and Engineering

Subscribe to Blog via Email

Enter your email address to subscribe to this blog and receive notifications of new posts by email.

Join 5,183 other subscribers

© 2025 Scienmag - Science Magazine

Welcome Back!

Login to your account below

Forgotten Password?

Retrieve your password

Please enter your username or email address to reset your password.

Log In
No Result
View All Result
  • HOME
  • SCIENCE NEWS
  • CONTACT US

© 2025 Scienmag - Science Magazine

Discover more from Science

Subscribe now to keep reading and get access to the full archive.

Continue reading