Sunlight, a fundamental source of life and well-being, plays a pivotal role in human health by enabling the synthesis of vitamin D, a critical nutrient for bone health and immune function. However, the beneficial rays that nourish us also harbor a darker facet: prolonged and unprotected exposure to ultraviolet (UV) radiation significantly escalates the risk of skin cancers, among the most common malignancies worldwide. This duality of UV radiation exposure—essential yet potentially deadly—has long baffled researchers seeking to unravel the molecular mechanisms that tip the balance from health to disease.
In a landmark study recently published in Nature Communications, a team of scientists from the University of Chicago has made a seminal breakthrough, elucidating how chronic UV exposure precipitates inflammation at the cellular level and propels the transformation of normal skin cells into malignant ones. Central to their discovery is a protein known as YTHDF2, a molecular gatekeeper whose degradation after UV damage opens the floodgates to harmful inflammation, setting the stage for carcinogenesis.
Skin cancers, including basal cell carcinoma and squamous cell carcinoma, account for over 5.4 million diagnosed cases annually in the United States alone, with over 90% directly linked to excessive UV radiation from sunlight. UV rays inflict DNA damage and provoke oxidative stress within skin cells, igniting inflammatory pathways responsible for the hallmark symptoms of sunburn: redness, blistering, and pain. Despite the ubiquity of these inflammatory responses, the fine-tuned molecular regulation that governs inflammation post-UV exposure has remained elusive—until now.
The investigative team, led by Professor Yu-Ying He, delved into the nuanced interactions between environmental stressors and cellular molecular machinery. Their focus centered on RNA metabolism, a critical nexus where small cellular molecules dictate gene expression and immune responses. RNA, or ribonucleic acid, typically translates genetic blueprints into functional proteins; however, a subset called non-coding RNAs, which do not encode proteins, plays intricate regulatory roles particularly within the cellular compartments of the nucleus and cytoplasm.
Through a series of rigorous experiments and multi-omics analyses, the researchers identified YTHDF2—a protein that selectively recognizes RNA sequences tagged with a chemical modification known as N6-methyladenosine (m6A)—as a pivotal moderator of the inflammatory cascade triggered by UV stress. Under normal conditions, YTHDF2 binds these m6A-marked RNA molecules, orchestrating their metabolism to maintain cellular homeostasis and dampen excessive inflammation. However, UV radiation triggers a notable depletion of YTHDF2 levels in skin cells, unleashing unchecked inflammatory responses.
Further inquiry revealed that YTHDF2 specifically interacts with a distinct non-coding RNA species, U6 small nuclear RNA (snRNA), which is also modified by m6A. U6 snRNA traditionally resides within the nucleus, facilitating RNA splicing and gene regulation. Surprisingly, under UV-induced stress, elevated levels of U6 snRNA translocate to endosomes—membrane-bound cellular compartments conventionally involved in material recycling—a remarkable mislocalization that contributes to pathological signaling.
This translocation is mediated by a protein named SDT2, which escorting U6 snRNA into the endosome, inadvertently drags along YTHDF2. Within the endosomal microenvironment, YTHDF2 exerts a suppressive effect, preventing U6 snRNA from aberrantly activating toll-like receptor 3 (TLR3), an immune sensor that upon stimulation ignites potent inflammatory pathways linked to tumorigenesis. The absence of YTHDF2 following UV exposure removes this inhibitory checkpoint, allowing U6 snRNA to engage TLR3 promiscuously, thereby exacerbating inflammatory damage and fostering a pro-carcinogenic milieu.
The study’s revelations unmask a previously unappreciated surveillance paradigm in cellular immunity and inflammation, positioning YTHDF2 as a crucial sentinel that curbs destructive inflammatory signaling triggered by self-RNA molecules. This intricate interplay between RNA modifications, protein readers, and immune sensors offers fresh mechanistic insights into how environmental insults like UV radiation transmute benign skin cells into malignant counterparts through dysregulated inflammation.
These findings not only augment our molecular understanding of skin cancer pathophysiology but also illuminate promising avenues for therapeutic innovation. By targeting the RNA-protein interactions and trafficking processes that underpin UV-induced inflammatory responses, novel interventions could be designed to bolster YTHDF2 function or mimic its regulatory role, thereby mitigating cancer risk. Such strategies might herald a new era in dermatological oncology, focusing on molecular precision to preemptively quell the inflammatory precursors of skin malignancies.
Moreover, this breakthrough prompts a revision of current paradigms regarding non-coding RNA dynamics and subcellular trafficking, expanding the landscape of RNA biology beyond canonical realms. The discovery that non-coding RNAs like U6 can aberrantly shuttle into endosomes to modulate innate immune receptors challenges long-held notions and invites further exploration into the cross-talk between RNA metabolism and immune regulation in diverse disease states.
Professor Yu-Ying He and colleagues emphasize that while inflammation is indispensable for combating infections and repairing tissue damage, its chronic or dysregulated activation is a double-edged sword that can precipitate oncogenic transformation. Their work deftly elucidates the molecular underpinnings that keep such inflammation in check under physiological conditions, revealing vulnerabilities exploited by UV-induced damage.
The research was supported by prominent institutions including the National Institutes of Health and the University of Chicago Medicine Comprehensive Cancer Center, underscoring the importance and high impact of this work. Collaborative contributions from a multidisciplinary team further highlight the integrative approach necessary to dissect complex cellular mechanisms influencing disease.
Together, these insights forge a vital link between environmental exposures, molecular regulatory systems, and cancer development, providing a blueprint for future research and clinical innovations aimed at safeguarding skin health. As ultraviolet radiation continues to pose a global health challenge, understanding and manipulating the molecular sentinels that guard against its deleterious effects may become key to reducing the burden of skin cancer worldwide.
Subject of Research: Cells
Article Title: YTHDF2 regulates self non-coding RNA metabolism to control inflammation and tumorigenesis
News Publication Date: 12-Nov-2025
Web References: https://www.nature.com/articles/s41467-025-64898-7
References: He, Y.-Y. et al. (2025). YTHDF2 regulates self non-coding RNA metabolism to control inflammation and tumorigenesis. Nature Communications. https://www.nature.com/articles/s41467-025-64898-7
Keywords: Cell biology, Cancer, Skin cancer, Ultraviolet radiation
