Social animals are strongly motived to seek out the company of others, especially after periods of isolation, because their brains are wired to find it rewarding. A study in mice published February 11 in Cell now reveals a neural circuit that mediates social seeking behavior driven instead by a loneliness-like state. By shedding light on the neuroscience of isolation, the findings could help our understanding of social anxiety and autism spectrum disorders.
"Our study is new in that a neural substrate for a loneliness-like state has never been identified before," says senior study author Kay Tye of the Massachusetts Institute of Technology. "There have been a number of studies examining social reward, but none that have looked at the negative motivational drive to seek social contact. In addition, we have identified a functional role for a population of neurons that were largely ignored and had never been directly tied to a functional role in motivated behavior."
This discovery came mostly by accident. Study co-authors Gillian Matthews and Mark Ungless of Imperial College London were studying the effects of cocaine on a neglected population of dopamine-releasing neurons in the dorsal raphe nucleus (DRN) of the brainstem. To their surprise, they noticed that the properties of these neurons changed when mice were separated from their cagemates, regardless of prior cocaine exposure. At that point, they realized that they needed to do a lot more work to lay the foundation for a completely new research area: the neuroadaptations of acute social isolation.
To follow up on this discovery, the researchers measured the activity of DRN dopamine neurons in mice that were either group-housed or socially isolated for 24 hours. Upon initial contact with a juvenile mouse, the mice that had been socially isolated showed an increase in the activity of DRN dopamine neurons compared with the group-housed mice. This finding suggests that these neurons respond to the reinforcing effects of social contact after isolation.
Tye and her team next used optogenetics to determine whether these neurons play a causal role in motivating social behavior. This technique involves genetically modifying specific populations of neurons to express light-sensitive proteins that control neural excitability and then delivering either blue or yellow light through an optic fiber to activate or inhibit those cells, respectively.
Activation of DRN dopamine neurons increased the preference of mice to spend more time on the side of a chamber containing another mouse rather than on the empty side of the chamber. By contrast, inhibition of DRN dopamine neurons decreased social seeking behavior in mice, but only after they had been socially isolated for 24 hours. The findings suggest that DRN dopamine neurons play a key role in driving social behavior in response to a loneliness-like state.
Remarkably, the degree to which these neurons modulated social behavior was predicted by social rank. In more dominant mice, stimulation of DRN dopamine neurons was more effective at promoting social preference. Moreover, inhibition of these neurons in more dominant mice caused a greater reduction in social preference following isolation.
"Social interactions may be more rewarding in dominant males because they have priority access to food and mates and tend to win territorial conflicts," Tye says. "As a result, dominant mice may experience a more pronounced loneliness-like state, increasing their drive to seek out social company after periods of isolation."
Taken together, the findings suggest that DRN dopamine neurons represent the subjective experience of a loneliness-like state. "However, we cannot assume that mice experience loneliness in the same way that humans do, and we can never assume to know the subjective emotional experience of a mouse. We can only look at the behavioral outputs," she cautions.
In future studies, the researchers will explore the inputs and outputs of these neurons, further examine the influence of social rank, and determine whether the results extend to non-social mammals. They also want to determine if there are any potential targets for helping people with social impairments, such as social anxiety or autism spectrum disorders.
This work was supported by the JPB Foundation, PIIF, PNDRF, JFDP, the Whitehall Foundation, the Klingenstein Foundation, a NARSAD Young Investigator Award, the Alfred P Sloan Foundation, the New York Stem Cell Foundation, the McKnight Foundation, a Whitehead Career Development Chair, NIMH, NIA, NIDDK, the UK Medical Research Council, the Royal Society, the Simons Center for the Social Brain at MIT, NSF, MIT, and Northeastern University.
Cell, Matthews, Nieh, and Vander Weele et al.: "Dorsal Raphe Dopamine Neurons Represent the Experience of Social Isolation" http://dx.doi.org/10.1016/j.cell.2015.12.040
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