Researchers conducted systematic field surveys and long-term in situ monitoring at the type locality of Boulenophrys elongata, located on Mt. Lianhua in Huidong County, Guangdong Province. The study area, situated at elevations between 700 and 1000 meters above sea level, features flowing montane streams and nearby forest floors within subtropical evergreen broad-leaved forests. By collecting five adult individuals during the breeding season, the team enabled the supplementation of morphological diagnoses and the establishment of baseline ecological data. These specimens, including three adult males and two adult females, were fixed in 10% buffered formalin and later transferred to 70% ethanol for detailed examination. The collection of these breeding individuals was crucial for clarifying the sexual dimorphism that had remained insufficiently clarified in previous studies, providing the first empirical support for population assessments and conservation strategies for this montane amphibian.

The morphological analysis revealed distinct sexual dimorphism that was absent in the original type series. Adult males measured between 27.8 and 30.2 mm in snout-vent length, while adult females ranged from 34.6 to 37.6 mm. Breeding males exhibited a nuptial pad with dense black nuptial spines at the bases of fingers I and II, a subgular vocal sac, and dense tiny conical spines on their dorsum. These traits, combined with the presence of vomerine ridges and teeth, and a well-developed canthus rostralis, helped refine the morphological diagnosis of the species. The dorsal skin was relatively smooth with dense granules and a weak discontinuous X-shaped ridge on the center of the dorsum, while the limbs featured discontinuous transverse ridges and tubercles. This detailed characterization resolves taxonomic ambiguity and provides a clearer framework for identifying the species in the field, distinguishing it from other members of the genus that often exhibit similar external features but differ in subtle reproductive traits.

Ecological observations indicated that Boulenophrys elongata is strictly associated with flowing montane streams and the adjacent forest floor. The breeding season appears to span from early March to late August, with reproductive activities triggered exclusively by heavy rainfall. Male advertisement calls were recorded during this period, but they ceased if rainfall stopped for several successive days, even under high ambient humidity. This dependence on specific weather conditions highlights the vulnerability of the species to climate variability and habitat fragmentation. The toads inhabit a microhabitat characterized by intricate narrow rock formations and dense shrubs, which provide shelter and breeding sites. Understanding these ecological traits is essential for conservation efforts, as the narrow range and specific habitat requirements make Boulenophrys elongata particularly susceptible to environmental changes and anthropogenic disturbances in the montane regions of southern China.

One of the most significant findings of this study is the first formal documentation of pre-amplexus reproductive behavior in the genus Boulenophrys. Continuous observation of the courtship behavior was carried out for 56 minutes on March 28, 2026, under minimal field disturbance. The sequence began with males establishing calling territories on riparian shrub foliage at approximately 25 cm above the ground. They produced intermittent quack-like advertisement calls via a single subgular vocal sac to attract conspecific females and defend their home ranges. The female, initially stretching its body forward on leaves, adjusted its orientation and crawled towards the male after detecting the calls. This initial interaction set the stage for a complex series of behavioral exchanges that demonstrated the intricate nature of mating strategies in this species.

The courtship process involved several distinct stages, including mutual behavioral recognition and positional adjustment. The male and female engaged in reciprocal blinking responses, which served as typical interactive cues during the assessment phase. The male would cease calling when the female blinked or turned its head, indicating a sensitive response to visual signals from the female. Physical contact occurred when the male touched the posterior margin of the female’s snout with its snout tip and rubbed the digits of the female’s fore-limb with its snout and eyelids. This tactile stimulation, accompanied by vocal signals and blinking, highlighted the multi-modal nature of communication between the sexes. The female’s responses, including head tilts and short jumps, played a crucial role in the progression of the courtship sequence, demonstrating a dynamic interaction that was previously undocumented in the genus.

Following the initial physical contact, the male shifted to a companion-leading courtship strategy, guiding the female towards potential breeding microhabitats. The male crawled steadily towards the direction of the advertisement calls, while the female followed by alternating crawling and jumping. The pair maintained close proximity, with seven intermittent physical contacts occurring throughout the movement. When separated, the male produced calls to re-orientate the female, who responded with a single blink after relocating the male. This guided movement led the pair towards a seepage area concealed within rock crevices, approximately 12 meters away. The observation ended before amplexus could be observed, but the detailed recording of the pre-copulatory sequence provided valuable insights into the mating behavior of Boulenophrys elongata and the broader genus.

The study underscores the importance of sustained field surveys and long-term monitoring for effective anuran conservation. The observed behavioral patterns, including male calling, female assessment, snout contact, and male-led guiding, suggest complex reproductive adaptations in fragmented montane habitats. However, limitations such as interrupted observations due to dense vegetation and rugged terrain, and the restriction to a single recorded event, highlight the need for repeated observations to confirm the consistency and generality of these patterns. The findings provide a comparative framework for behavioral evolution and phylogenetic studies within the genus, emphasizing the value of targeted fieldwork in unraveling the diversified reproductive behavior of Boulenophrys species. This research not only resolves taxonomic ambiguities but also establishes a baseline for future conservation assessments and population monitoring of this narrow-range endemic species.

Subject of Research: Reproductive behavior and sexual dimorphism in Boulenophrys elongata

Article Title: Supplementary description of Boulenophrys elongata ( Anura , Megophryidae ): Sexual dimorphism and observations on reproductive behaviours

Article References: Wang, J., Zhan, B.-B., Tan, L., Xiao, W.-W., Li, Y.-H., Lin, S.-S., & Zeng, Z.-C. (2026). Supplementary description of Boulenophrys elongata (Anura, Megophryidae): Sexual dimorphism and observations on reproductive behaviours. Biodiversity Data Journal, 14, Article e201422. https://doi.org/10.3897/bdj.14.e201422

Image Credits: AI Generated

DOI: 10.3897/BDJ.14.e201422

Keywords: Boulenophrys elongata, sexual dimorphism, reproductive behavior, amphibian conservation, montane streams, courtship ritual, herpetology, Guangdong, horned toads, ecological monitoring, Supplementary, description