The experimental system was built on Caco-2 cells, a workhorse of gastrointestinal research. When grown under the right conditions, these human colorectal cancer cells differentiate into a monolayer that behaves remarkably like the lining of the small intestine, complete with absorptive properties, tight junctions between neighboring cells, and even the capacity to secrete enteroendocrine hormones. That makes them an ideal first-pass model for asking whether a botanical extract can influence the biology of the gut wall before any animal or human work is attempted. The team, led by Heggar Venkataramana Sudeep, first established a safety window using an MTT cytotoxicity assay, testing concentrations of the extract ranging from 25 to 500 micrograms per milliliter. Only concentrations that left the cells unharmed were carried forward, ensuring that any molecular changes observed were genuine regulatory effects rather than signs of cellular distress.

The headline finding concerns the GLP-1 system itself. After 24 hours of treatment, the highest non-toxic dose, 100 micrograms per milliliter, drove a 3.33-fold increase in the expression of the Proglucagon gene, the blueprint from which GLP-1 is manufactured, and a 3.32-fold rise in the gene encoding the GLP-1 receptor, the molecular dock that receives the hormone’s signal. Both changes were statistically robust, with the Proglucagon effect reaching a significance threshold of p less than 0.001. The paired upregulation is significant because it suggests the extract is not merely prompting cells to produce more hormone but also priming the signaling apparatus that responds to it, a combination the authors interpret as stimulation of endogenous GLP-1 signaling rather than a one-dimensional hormonal push.

Producing more GLP-1 is only half the battle, because the hormone is famously short-lived. An enzyme called dipeptidyl peptidase-4, or DPP-4, slices incretin hormones into inactive fragments within minutes of their release, which is precisely why a class of diabetes drugs called DPP-4 inhibitors exists to prolong GLP-1’s working life. The extract appeared to attack this problem from two directions. In the Caco-2 cells, Leanskolin significantly reduced both the activity of cellular DPP-4 and the expression of the gene that encodes it, compared with untreated controls. In a separate cell-free enzyme assay, the extract inhibited DPP-4 directly, with a half-maximal inhibitory concentration, or IC50, of 262.5 micrograms per milliliter. That potency is modest compared with synthetic pharmaceutical inhibitors, but the dual mechanism, suppressing the enzyme’s production while also dampening its activity, hints at a layered strategy for extending the bioavailability of the hormone the cells are producing in greater quantities.

Perhaps the most intriguing piece of the puzzle involves bitter taste receptors. It is now well established that the gut is not a passive tube but a sensory organ, studded with the same TAS2R bitter receptors found on the tongue. When these receptors detect bitter compounds, they can trigger cascades that influence appetite, hormone release, and gut motility. In the study, Leanskolin modulated the expression of three bitter receptor genes, TAS2R38, TAS2R43, and TAS2R14, in a concentration-dependent manner with statistical significance at p less than 0.05. The authors suggest this points to a role in nutrient sensing and enteroendocrine signaling, raising the possibility that forskolin or companion compounds in the extract act as bitter agonists that help coax the gut’s hormone-producing cells into action. If confirmed, this would place the extract in a growing family of bitter-tasting botanicals being investigated as natural modulators of gut hormone release.

The second major axis of the study concerned the physical integrity of the intestinal barrier. A healthy gut wall is a selectively permeable gatekeeper, held together by tight junction proteins that seal the gaps between epithelial cells. When that seal loosens, a condition sometimes called leaky gut, bacterial fragments and other unwanted molecules can slip into the bloodstream, fueling the low-grade inflammation that is increasingly implicated in obesity, insulin resistance, and metabolic syndrome. The researchers assessed barrier function using two complementary readouts. Transepithelial electrical resistance, or TEER, measures how well the cell monolayer resists the passage of ions, while FITC-flux assays track the movement of a fluorescent marker across the layer, a proxy for paracellular leakiness.

The results here were nuanced but encouraging. Treatment with the extract at concentrations from 25 to 100 micrograms per milliliter for 24 hours did not change TEER values, suggesting the overall ionic tightness of the monolayer was preserved rather than perturbed. However, FITC-flux dropped significantly at the 50 and 100 microgram doses, with p values below 0.01, indicating that the passage of larger molecules through the paracellular route was reduced. Consistent with that functional improvement, the extract enhanced the expression of tight junction proteins, the molecular rivets of the barrier, at p less than 0.05 compared with controls. In other words, the barrier was not merely being stressed into tightening; the cells appeared to be actively building more of the structural proteins that hold the wall together.

Taken together, the authors frame the extract as a multi-target agent for metabolic health, simultaneously activating GLP-1 pathway genes, curbing the enzyme that degrades GLP-1, engaging the sensory receptors that regulate enteroendocrine responses, and reinforcing the gut’s physical defenses. That combination matters because these processes are biologically intertwined. GLP-1 released from intestinal L-cells slows gastric emptying, promotes satiety, and enhances glucose-dependent insulin secretion, while a robust epithelial barrier limits the inflammatory signals that can drive insulin resistance. A single intervention that touches both sides of that equation is unusual, particularly one derived from a plant whose active constituent has been on the market for decades.

Caution is warranted before anyone reaches for a forskolin supplement expecting a natural Ozempic. This was a cell culture study, and the concentrations used, up to 100 micrograms per milliliter applied directly to cells in a dish, do not translate straightforwardly into human doses or blood levels. Caco-2 cells, though useful, are a cancer-derived model that only approximates real intestinal physiology, and gene expression changes do not guarantee meaningful hormone secretion in a living body. There is also a disclosure worth noting: all of the authors are employed by Vidya Herbs Pvt. Ltd., the company behind the Leanskolin ingredient, and the paper explicitly declares this potential conflict of interest. Independent replication in animal models and eventually controlled human trials will be essential to determine whether the effects survive the journey from dish to person.

Even with those caveats, the study opens a genuinely novel research direction. Forskolin has historically been studied for its ability to activate adenylate cyclase and raise intracellular cyclic AMP, a signaling molecule with wide-reaching effects, and it is tempting to speculate that this canonical mechanism could underlie some of the observed gene expression changes. But the breadth of the effects, spanning incretin genes, DPP-4, bitter receptors, and tight junction proteins, suggests the standardized extract may act through converging pathways rather than a single switch. As interest in gut-derived hormones reshapes metabolic medicine, the idea that a well-characterized botanical extract could engage the same biology, however gently, is likely to keep this line of investigation moving from the culture flask toward the clinic.

Subject of Research: Effects of a standardized Coleus forskohlii extract on GLP-1 signaling and intestinal barrier integrity in Caco-2 cells

Article Title: Targeting GLP-1 signaling and barrier integrity with Leanskolin™, a standardized Coleus forskohlii extract: a Caco-2 cell-based study for metabolic health support

Article References: Sudeep, H. V., Kumara, T. P. P., Lingaraju, H. B., & Shyamprasad, K. (2026). Targeting GLP-1 signaling and barrier integrity with Leanskolin™, a standardized Coleus forskohlii extract: a Caco-2 cell-based study for metabolic health support. BMC Complementary Medicine and Therapies. https://doi.org/10.1186/s12906-026-05590-1

Image Credits: AI Generated

DOI: 10.1186/s12906-026-05590-1

Keywords: GLP-1, forskolin, Coleus forskohlii, Caco-2 cells, DPP-4 inhibition, gut barrier, tight junctions, incretin hormones, metabolic health, bitter taste receptors, natural products, enteroendocrine signaling