UPTON, NY-New close-up images of the proteins that copy DNA inside the nucleus of a cell have led a team of scientists from the U.S. Department of Energy's Brookhaven National Laboratory, Stony Brook University, Rockefeller University, and the University of Texas to propose a brand new mechanism for how this molecular machinery works. The scientists studied proteins from yeast cells, which share many features with the cells of complex organisms such as humans, and could offer new insight into ways that DNA replication can go awry.
"DNA replication is a major source of errors that can lead to cancer," explained Huilin Li, a biologist with a joint appointment at Brookhaven Lab and Stony Brook University and the lead author on a paper describing the new results in Nature Structural & Molecular Biology. "The entire genome-all 46 chromosomes-gets replicated every few hours in dividing human cells," Li said, "so studying the details of how this process works may help us understand how errors occur."
The research builds on previous work by Li and others, including last year's collaboration with the same team that produced the first-ever images of the complete DNA-copying protein complex, called the replisome. That study revealed a surprise about the location of the DNA-copying enzymes-DNA polymerases. This new study zooms in on the atomic-level details of the "helicase" portion of the protein complex-the part that encircles and splits the DNA double helix so the polymerases can synthesize two daughter strands by copying from the two separated parental strands of the "twisted ladder."
The scientists produced high-resolution images of the helicase using a technique known as cryo-electron microscopy (cryo-EM). One advantage of this method is that the proteins can be studied in solution, which is how they exist in the cells.
"You don't have to produce crystals that would lock the proteins in one position," Li said. That's important because the helicase is a molecular "machine" made of 11 connected proteins that must be flexible to work. "You have to be able to see how the molecule moves to understand its function," Li said.
Using computer software to sort out the images revealed that the helicase has two distinct conformations-one with components stacked in a compact way, and one where part of the structure is tilted relative to a more "fixed" base.
The atomic-level view allowed the scientists to map out the locations of the individual amino acids that make up the helicase complex in each conformation. Then, combining those maps with existing biochemical knowledge, they came up with a mechanism for how the helicase works.
"One part binds and releases energy from a molecule called ATP. It converts the chemical energy into a mechanical force that changes the shape of the helicase," Li said. After kicking out the spent ATP, the helicase complex goes back to its original shape so a new ATP molecule can come in and start the process again.
"It looks and operates similar to an old style pumpjack oil rig, with one part of the protein complex forming a stable platform, and another part rocking back and forth," Li said. Each rocking motion could nudge the DNA strands apart and move the helicase along the double helix in a linear fashion, he suggested.
This linear translocation mechanism appears to be quite different from the way helicases are thought to operate in more primitive organisms such as bacteria, where the entire complex is believed to rotate around the DNA, Li said. But there is some biochemical evidence to support the idea of linear motion, including the fact that the helicase can still function even when the ATP hydrolysis activity of some, but not all, of the components is knocked out by mutation.
"We acknowledge that this proposal may be controversial and it is not really proven at this point, but the structure gives an indication of how this protein complex works and we are trying to make sense of it," he said.
The study was funded by the U.S. National Institutes of Health and the Howard Hughes Medical Institute (HHMI), with additional support from the Brookhaven Lab Biology Department. High-resolution cryo-EM data were collected at HHMI and the University of Texas Health Science Center.
An electronic version of this news release with related multimedia content
Scientific paper: "Structure of the eukaryotic replicative CMG helicase suggests a pumpjack motion for translocation"
Press releases on previous related work:
First Complete Pictures of Cells' DNA-Copying Machinery
Scientists Map Key Moment in Assembly of DNA-Splitting Molecular Machine
Key Step in Molecular 'Dance' that Duplicates DNA Deciphered
Study Reveals How Protein Machinery Binds and Wraps DNA to Start Replication
Brookhaven National Laboratory is supported by the Office of Science of the U.S. Department of Energy. The Office of Science is the single largest supporter of basic research in the physical sciences in the United States, and is working to address some of the most pressing challenges of our time. For more information, please visit science.energy.gov.
One of ten national laboratories overseen and primarily funded by the Office of Science of the U.S. Department of Energy (DOE), Brookhaven National Laboratory conducts research in the physical, biomedical, and environmental sciences, as well as in energy technologies and national security. Brookhaven Lab also builds and operates major scientific facilities available to university, industry and government researchers. Brookhaven is operated and managed for DOE's Office of Science by Brookhaven Science Associates, a limited-liability company founded by the Research Foundation for the State University of New York on behalf of Stony Brook University, the largest academic user of Laboratory facilities, and Battelle, a nonprofit applied science and technology organization.
Karen McNulty Walsh