Organic insect deterrent for agriculture

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Credit: Wolfgang Mischko / TUM

Traditional insecticides are killers: they not only kill pests, they also endanger bees and other beneficial insects, as well as affecting biodiversity in soils, lakes, rivers and seas. A team from the Technical University of Munich (TUM) has now developed an alternative: A biodegradable agent that keeps pests at bay without poisoning them.

"It's not just about the bees, it's about the survival of humanity," says Professor Thomas Brück, who heads the Werner Siemens Chair of Synthetic Biotechnology at TU Munich. "Without the bees that pollinate a wide variety of plants, not only would our supermarket shelves be quite bare, but within a short time, it would no longer be possible to supply the world's population with food."

Synthetically produced insecticides endanger not only bees but also beetles, butterflies and grasshoppers. They affect biodiversity in soils, lakes, rivers and seas. Their use has consequently been highly controversial for many years.

Repelling instead of poisoning

Brück and his team have now found an alternative: The insect repellent they have developed is biodegradable and ecologically harmless. Sprayed on plants, it works much like mosquito repellent used by bathers in the summer, spreading a smell that keeps away unwanted insects.

"With our approach, we are opening the door to a fundamental change in crop protection," says Brück. "Instead of spraying poison, which inevitably also endangers useful species, we deliberately merely aggravate the pests."

Bacteria as chemical factories

The Munich researchers were inspired by the tobacco plant, which produces cembratrienol in its leaves, CBTol for short. The plant uses this molecule to protect itself from pests.

Using synthetic biotechnology tools, Professor Brück's team isolated the sections of the tobacco plant genome responsible for the formation of the CBTol molecules. They then built these into the genome of coli bacteria. Fed with wheat bran, a by-product from grain mills, the genetically modified bacteria now produce the desired active agent.

Efficiency in small and large scales

"The key challenge during production was to separate the active ingredients from the nutrient solution at the end of the process," explains Mirjana Minceva, Professor of Biothermodynamics at the TUM Weihenstephan Campus.

The solution was centrifugal separation chromatography: a highly efficient process that works equally well on an industrial scale, but hitherto had never been used to separate products from fermentation processes.

Equally effective against bacteria

Initial investigations indicate that the CBTol spray is non-toxic to insects, yet still protects against aphids. Since it is biodegradable, it does not accumulate.

In addition, the bioactivity tests showed that cembratrienol has an antibacterial effect on gram-positive bacteria. It can thus be used as a disinfectant spray that acts specifically against pathogens such as Staphylococcus aureus (MRSA pathogen), Streptococcus pneumoniae (pneumonia pathogen) or Listeria monocytogenes (listeriosis pathogen).

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The project was funded by the Bavarian Ministry of Economic Affairs, Energy and Technology and the Werner Siemens Foundation. The research team used the equipment pool of the TUM Center for Industrial Biotechnology in Garching to do the technical scaling and validation of the cembratrienol production. The bioactivity tests were carried out in the Department of Biochemical Engineering of the Friedrich Alexander University Erlangen-Nürnberg.

Publication

Wolfgang Mischko, Max Hirte, Simon Roehrer, Hannes Engelhardt, Norbert Mehlmer, Mirjana Minceva and Thomas Brück

Modular Biomanufacturing for a Sustainable Production of Terpenoid-based Insect Deterrents

Green Chemistry, May 14, 2018 – DOI: 10.1039/C8GC00434J

Media Contact

Dr. Andreas Battenberg
[email protected]
49-892-891-0510
@TU_Muenchen

http://www.tum.de

       <h4>Original Source</h4>https://www.tum.de/nc/en/about-tum/news/press-releases/detail/article/34679/ http://dx.doi.org/10.1039/C8GC00434J 
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