Science news and articles on health, environment, global warming, stem cells, bird flu, autism, nanotechnology, dinosaurs, evolution -- the latest discoveries in astronomy, anthropology, biology, chemistry, climate & bioengineering, computers, engineering ; medicine, math, physics, psychology, technology, and more from the world's leading research centers universities.

Initiating DNA repair

0

To find out what activates SIRT6, the researchers alternately applied chemical inhibitors to human skin cells to determine which proteins were essential in getting the gene to repair the broken DNA strands. They discovered that one protein was involved in activating the gene in response to oxidative stress–c-Jun N-terminal kinase, which goes by the simpler term JNK. When JNK was inhibited, SIRT6 was not activated and the broken strands of DNA were not repaired efficiently.

The findings have been published in the journal Cell Reports.

To communicate stress signals within cells, JNKs add phosphate groups to proteins, and the Rochester study found the amino acid residue on SIRT6 that is modified by JNK. Once modified, SIRT6 can attract the enzyme PARP1 (Poly [ADP-ribose] polymerase 1) to the damaged site, where the enzyme undertakes a chemical process to repair the DNA. In effect, the activated gene serves as a first responder, recruiting DNA repair enzymes to the accident site and getting them to work.

The study is the latest work by Gorbunova and Seluanov to shed light on the molecular mechanisms that drive the aging process. Their previous work involved understanding the prominence of an inferior DNA repair process later in life, as well as how errant DNA fragments–called jumping genes–are typically kept inactive.

Understanding the molecular, chemical, and genetic process of aging has implications for both longevity and quality of life. While more research and clinical work need to be done, such studies help pave the way for possible treatments in the future.

For example, Seluanov says the results may allow pharmaceutical researchers to one day design drugs that activate SIRT6 in ways that reduce molecular damage.

"These drugs may be used to protect our genomes from damage, and could ultimately prevent cancer and extend healthy lifespan," he says.

###

Media Contact

Peter Iglinski
[email protected]
585-273-4726
@UofR

http://www.rochester.edu

Leave A Reply

Your email address will not be published.