Forthcoming comprehensive reference guide on deep tissue imaging techniques

Innovations in novel optical imaging techniques promise noninvasive deep tissue diagnostic tools that are more powerful and less expensive than existing modalities. A forthcoming reference book for new deep tissue imaging techniques, to be published January 31, 2017 by Pan Stanford, provides a comprehensive guide for researchers and students of multiple disciplines.

Deep Imaging in Tissue and Tissue-Like Media with Linear and Nonlinear Optics is edited by two leaders: one in the field of ultrafast laser spectroscopy–Dr. Robert R. Alfano of the City College of the City University of New York, and other in field of multiphoton imaging–Dr. Lingyan Shi of Columbia University.

This book provides an overview of emerging novel optical imaging techniques, Gaussian beam optics, light scattering, nonlinear optics, and nonlinear optical tomography of tissues and cells. It consists of pioneering works that employ different linear and nonlinear optical imaging techniques for deep tissue imaging, including the new applications of single- and multiphoton excitation fluorescence, Raman scattering, resonance Raman spectroscopy, second harmonic generation, stimulated Raman scattering gain and loss, coherent anti-Stokes Raman spectroscopy, and NIR, SWIR , and MIR supercontinuum spectroscopy.

Dr. Alfano said, "the use of the photon basic unit of light offers an unique opportunity via its many properties to understand various states of matter on more fundamental level. Shi and I believe biomedphotonics will open the door to the underlying mysteries of the brain and cancer."

The use of light for probing and imaging biomedical media is promising for the development of safe, noninvasive, and inexpensive clinical imaging modalities with diagnostic ability of cancer and brain disorders. The advent of ultrafast lasers has enabled applications of nonlinear optical processes, which allow for deeper imaging in biological tissues with higher spatial resolution.

Robert R. Alfano is a distinguished professor of science engineering at the City College of the City University of New York. He received his PhD in physics from New York University and worked at GTE Laboratories (Verizon). He has pioneered many applications of light and photonics technology to the study of biological, biomedical, and condensed matter systems using optical spectroscopy and optical imaging. He has also discovered and used supercontinuum and novel tunable Cr solid state lasers. Dr. Alfano is a fellow of APS, OSA, and IEEE.

Lingyan Shi is a research scientist at Columbia University. Her current research focuses on metabolic imaging with stimulated Raman scattering microscopy. Before joining Columbia University, she was a research associate studying deep brain imaging and drug delivery in the brain at the Institute for Ultrafast Spectroscopy and Lasers Biology ,and Biomedical Engineering Departments at City College of New York (CCNY). Dr. Shi received her PhD in biomedical engineering from CCNY.


The City University of New York is the nation's leading urban public university. Founded in New York City in 1847, the University comprises 24 institutions: 11 senior colleges, seven community colleges, and additional professional schools. The University serves nearly 275,000 degree-credit students and 218,083 adult, continuing and professional education students.

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