FAU, collaborators receive $1.68 million NIH grant for sleep and metabolic disorders
A neuroscientist from Florida Atlantic University's Jupiter Life Science Initiative (JLSI) and collaborators from the University of Minnesota and Stowers Institute for Medical Research recently a received a four-year, $1.68 million R01 research grant from the National Institutes of Health's (NIH) National Institute of General Medical Sciences (NIGMS) for a project titled, "Understanding Complex Trait Architecture through Population Genomics."
Alex Keene, Ph.D., an associate professor in FAU's Charles E. Schmidt College of Science and a JLSI faculty member, is working together with fellow principal investigators, Suzanne McGaugh, Ph.D., an assistant professor in the College of Biological Sciences at the University of Minnesota, and Nicolas Rohner, Ph.D., an assistant investigator at Stowers Institute for Medical Research, on this collaborative project that utilizes genomic and transgenic technology in Mexican cavefish (Astyanax mexicanus) to identify genetic loci that contribute to sleep, feeding, and metabolism.
"The Mexican cavefish exhibits a dramatic evolution of sleep loss, hyperphagia or overeating, and obesity compared to a surface fish of the same species," said Keene. "They provide a unique and powerful model for identifying genetic factors regulating these traits."
As a result of living in total and permanent darkness in a small location in northeast Mexico, the eyeless, tiny blind Mexican cavefish has evolved sleeplessness, requiring far less sleep than their river-dwelling relatives. These fish have been studied for nearly 100 years for their fascinating traits, yet little is known about how their behavior evolves. Because of their lack of sleep, they provide a great model to study human sleep disorders such as insomnia.
"Because the cave environment differs dramatically from the rivers inhabited by surface fish, cavefish have evolved robust differences in foraging and feeding behavior, raising the possibility that differences in nutrient availability contribute to the evolution of sleep loss in cave populations," said Keene. "Furthermore, multiple cave populations have evolved substantial reductions in sleep duration and enhanced sensory systems, and our prior research suggests that sleep loss is evolutionary and functionally associated with sensory and metabolic changes."
There are more than 29 different populations of cavefish and many of them evolved independently. Keene and his research team have generated transgenic fish lines and are able to image brain activity and genetically map anatomical differences between these Mexican cavefish populations.
"Sleep and metabolic disorders present an enormous health burden globally and investigating the genes and neural circuits that regulate sleep loss will help to shed light on the interaction between insomnia, obesity, and metabolic disorders," said Keene. "In addition, this research project has the potential to identify whether shared genetic architecture underlies changes in these traits, providing insight into the relationship between sleep loss and metabolic disorders."
Beyond their immediate scientific goals, Keene, McGaugh and Rohner will establish transgenic and genomic tools and techniques to provide a valuable resource that can be applied to address questions beyond the scope of the grant project, including investigating eye-degeneration and autism.
JLSI is aimed at building educational and research capabilities in the life sciences on FAU's John D. MacArthur Campus in Jupiter, and is a collaborative effort between the Charles E. Schmidt College of Science and the Harriet L. Wilkes Honors College.
About Florida Atlantic University:
Florida Atlantic University, established in 1961, officially opened its doors in 1964 as the fifth public university in Florida. Today, the University, with an annual economic impact of $6.3 billion, serves more than 30,000 undergraduate and graduate students at sites throughout its six-county service region in southeast Florida. FAU's world-class teaching and research faculty serves students through 10 colleges: the Dorothy F. Schmidt College of Arts and Letters, the College of Business, the College for Design and Social Inquiry, the College of Education, the College of Engineering and Computer Science, the Graduate College, the Harriet L. Wilkes Honors College, the Charles E. Schmidt College of Medicine, the Christine E. Lynn College of Nursing and the Charles E. Schmidt College of Science. FAU is ranked as a High Research Activity institution by the Carnegie Foundation for the Advancement of Teaching. The University is placing special focus on the rapid development of critical areas that form the basis of its strategic plan: Healthy aging, biotech, coastal and marine issues, neuroscience, regenerative medicine, informatics, lifespan and the environment. These areas provide opportunities for faculty and students to build upon FAU's existing strengths in research and scholarship. For more information, visit http://www.fau.edu.