Crystal structure reveals new details of nonstandard RNA transcription


Credit: Murakami laboratory, Penn State University

Out through the window: Crystal structure reveals details of nonstandard RNA transcription

University Park, PA — High-resolution crystal structure reveals a new pathway for RNA during a nontraditional form of transcription — the process by which RNA is produced from a DNA template. Caught during the act of reiterative transcription, a form of transcription in which a single base of DNA (represented by the letters A, T, C, and G) codes for several corresponding bases in the RNA (one G in DNA leads to several Gs in the RNA, for example), the new crystal structure reveals RNA exiting the polymerase enzyme through an alternative channel to enable this unconventional mode of transcription. A paper describing the findings by a team of researchers at Penn State University and the University of Alabama at Birmingham appears in the journal Proceedings of the National Academy of Sciences.

"In the classical view of X-ray crystallography, you would think that the enzyme — RNA polymerase — would be inactivated and we couldn't capture a biochemical process in action in a crystallized state," said Katsuhiko S. Murakami, professor of biochemistry and molecular biology at Penn State and an author of the paper. "But in some systems, including ours, the RNA polymerase is still alive, so we can monitor the structures while they are in the process of making RNA."

Reiterative transcription was discovered by Penn State alumnus and Nobel Laureate in chemistry Paul Berg, the Robert W. and Vivian K. Cahill Professor of Cancer Research, Emeritus, at Stanford University, in the early 1960s. Although it could appear to be a simple error in accurately transcribing the DNA sequence into RNA — reiterative transcription occurs when the strand of RNA slips relative to the strand of DNA inside the polymerase enzyme — it has function in the cell. Reiterative transcription plays a key role in controlling gene expression for many genes, but despite this important role, its mechanism has remained a black box.

"Our work," said Vadim Molodtsov, a research associate at Penn State and an author of the paper. "By capturing the crystal structure of RNA polymerase during reiterative transcription, we were able to identify a new pathway used by RNA to exit the enzyme. Instead of leaving through the door, it sneaks out the window."


In addition to Murakami and Molodtsov, the research team includes Yeonoh Shin, a graduate student in the Penn State Biochemistry, Microbiology, and Molecular Biology Program, and Charles Turnbough, Jr., emeritus professor of microbiology at the University of Alabama at Birmingham. The research was funded by the U.S. National Institute of General Medical Science.


Katsuhiko Murakami:
[email protected]

Barbara K. Kennedy
[email protected]



Schematic of alternative pathways for transcription elongation complex formation. During canonical transcription (left) the growing strand of RNA extends toward the RNA exit channel of RNA polymerase. New, high-resolution crystal structure determined in this study shows that during reiterative transcription (right) the RNA extends toward the main channel of RNA polymerase.

FIGURE CREDIT: Murakami laboratory, Penn State University

Media Contact

Barbara K. Kennedy
[email protected]

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